성견 치주인대세포의 줄기세포 특성 연구
Stem cell properties of cells derived from canine periodontal Ligament
김경화, 설양조, 이용무, 김수환,
소속 상세정보
김경화 ( Kim Kyoung-Hwa ) - 서울대학교 치과대학 치주과학교실
설양조 ( Seol Yang-Jo ) - 서울대학교 치과대학 치주과학교실
이용무 ( Lee Yong-Moo ) - 서울대학교 치과대학 치주과학교실
김수환 ( Kim Su-Hwan ) - 서울대학교 치과대학 치주과학교실
KMID : 0363020070370030479
Abstract
In spite of the attention given to the study of mesenchymal stem cells derived periodontal ligament (PDL), there is a lack of information about canine PDL cells. In this study, we characterized canine PDL cells to clarify their stem cell properties, including self renewal, proliferate rate, stem cell markers and multipotency. PDL cells were obtained from extracted premolars of canines, following a colony forming assay and proliferation rate of sub-confluent cultures of cells for self-renewal, immunostaining for STRO-1 and CD146/MUC18 and a differentiation assay for multipotency. Canine PDL cells formed single-cells colonies and 25% of the PDL cells displayed positive staining for BrdU. The cells expressed the mesenchymal stem-cell markers, STRO-1 and CD146/MUC18. Under defined culture conditions, the cells differentiated into osteoblasts and adipocytes, but the cells didn’t differentiated into chondrocytes. The findings of this study indicated that the canine PDL cells possess crucial stem cells properties, such as self-renewal and multipotency, and express the mesenchymal stem cell markers on their surface. The isolation and characterization of canine PDL cells makes it feasible to pursue preclinical models of periodontal regeneration in canine.
키워드
Canine;mesenchymal stem cells;PDL;multipotency;STRO-1;CD146/MCC18
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